Abstract

Pectin, a heteropolysaccharide commercially derived from the cell wall of higher plants, is mainly used in food as a gelling agent in jams and jellies as well as a stabilizer in fruit juice and milk drinks. It has also received great interest as a source of dietary fiber. Furthermore, pectin is proved to have diverse biological activities including lipid and cholesterol level lowering effects, serum glucose and insulin content lowering effects, gastric emptying delay, and anti-cancer activities. Pectin and pectic oligosaccharides have been shown to induce apoptosis in human colonic adenocarcinoma cells and to have anti-metastatic properties. Dietary pectin can bind metal ions, particularly lead ions, thus reducing their retention in the body and diminishing their toxic effects. On the other hand, pectin enhances intestinal solubility and absorption of ferric iron. Pectin with a low degree of esterification or having a large volume of linear oligogalacturonide segments shows significant mucoadhesion capacity in the gastrointestinal tract. In this way pectin forms a physical barrier protecting epithelium against opportunistic microbial invasion during stress.

References

• 1. Avivi-Green C., Polak-Charcon S., Madar Z., Schwartz B.: Apoptosiscascade proteins are regulated in vivo by high intracolonicbutyrate concentration: correlation with colon cancer inhibition.Oncol. Res., 2000; 12: 83-95
  Google Scholar
• 2. Caffall K.H., Mohnen D.: The structure, function, and biosynthesisof plant cell wall pectic polysaccharides. Carbohydr. Res., 2009;344: 1879-1900
  Google Scholar
• 3. Campbell J.M., Fahey G.C.: Psyllium and methylocellulose fermentationproperties in relation to insoluble and soluble fiber standards.Nutr. Res., 1997; 17: 691-629
  Google Scholar
• 4. Chauhan D., Li G., Podar K., Hideshima T., Neri P., He D., MitsiadesN., Richardson P., Chang Y., Schindler J., Carver B., Anderson K.C.:A novel carbohydrate-based therapeutic GCS-100 overcomes bortezomibresistance and enhances dexamethasone-induced apoptosisin multiple myeloma cells. Cancer Res., 2005; 65: 8350-8358
  Google Scholar
• 5. Chen C.H., Sheu M.T., Chen T.F., Wang Y.C., Hou W.C., Liu D.Z.,Chung T.C., Liang Y.C.: Suppression of endotoxin-induced proinflammatoryresponses by citrus pectin through blocking LPS signalingpathways. Biochem. Pharmacol., 2006; 72: 1001-1009
  Google Scholar
• 6. Dongowski G., Lorenz A.: Intestinal steroids in rats are influencedby the structural parameters of pectin. J. Nutr. Biochem.,2004; 15: 196-205
  Google Scholar
• 7. Dongowski G., Lorenz A., Proll J.: The degree of methylation influencesthe degradation of pectin in the intestinal tract of rats andin vitro. J. Nutr., 2002; 132: 1935-1944
  Google Scholar
• 8. Feltrin C., Batista de Morais M., de Cássia Freitas K., Beninga deMorais T., Fagundes Neto U., Silvério Amancio O.M.: Effect of solublefiber pectin on growth and intestinal iron absorption in rats duringrecovery from iron deficiency anemia. Biol. Trace Elem. Res.,2009; 129: 221-228
  Google Scholar
• 9. Flourie B., Vidon N., Florent C.H., Bernier J.J.: Effect of pectin onjejunal glucose absorption and unstirred layer thickness in normalman. Gut, 1984; 25: 936-941
  Google Scholar
• 10. Fukumoto S., Tatewaki M., Yamada T., Fujimiya M., Mantyh C.,Voss M., Eubanks S., Harris M., Pappas T.N., Takahashi T.: Shortchainfatty acids stimulate colonic transit via intraluminal 5-HTrelease in rats. Am. J. Physiol. Regul. Integr. Comp. Physiol. , 2003;284: R1269-R1276
  Google Scholar
• 11. Fuse K., Bamba T., Hosoda S.: Effects of pectin on fatty acid andglucose absorption and on thickness of unstirred water layer in ratand human intestine. Dig. Dis. Sci., 1989; 34: 1109-1116
  Google Scholar
• 12. Glinsky V.V., Raz A.: Modified citrus pectin anti-metastaticproperties: one bullet, multiple targets. Carbohydr. Res., 2009; 344:1788-1791
  Google Scholar
• 13. Gulfi M., Arrigoni E., Amado R.: The chemical characteristicsof apple pectin influence its fermentability in vitro. LWT, 2006; 39:1001-1004
  Google Scholar
• 14. Gulfi M., Arrigoni E., Amado R.: In vitro fermentability of pectinfraction rich in hairy regions. Carbohydr. Polym., 2007; 67: 410-416
  Google Scholar
• 15. Gunness P., Gidley M.J.: Mechanisms underlying the cholesterolloweringproperties of soluble dietary fibre polysaccharides. FoodFunct., 2010; 1: 149-155
  Google Scholar
• 16. Gunning A.P., Bongaerts R.J., Morris V.J.: Recognition of galactancomponents of pectin by galectin-3. FASEB J., 2009; 23: 415-424
  Google Scholar
• 17. Holt S., Heading R.C., Carter D.C., Prescott L.F., Tothill P.: Effectof gel fibre on gastric emptying and absorption of glucose andparacetamol. Lancet, 1979; 1: 636-639
  Google Scholar
• 18. Inngjerdingen K.T., Langerud B.K., Rasmussen H., Olsen T.K.,Austarheim I., Grønhaug T.E., Aaberge I.S., Diallo D., Paulsen B.S.,Michaelsen T.E.: Pectic polysaccharides isolated from Malian medicinalplants protect against Streptococcus pneumoniae in a mousepneumococcal infection model. Scand. J. Immunol., 2013; 77: 372-388
  Google Scholar
• 19. Inngjerdingen M., Inngjerdingen K.T., Patel T.R., Allen S., ChenX., Rolstad B., Morris G.A., Harding S.E., Michaelsen T.E., Diallo D.,Paulsen B.S.: Pectic polysaccharides from Biophytum petersianumKlotzsch, and their activation of macrophages and dendritic cells.Glycobiology, 2008; 18: 1074-1084
  Google Scholar
• 20. Jackson C.L., Dreaden T.M., Theobald L.K., Tran N.M., Beal T.L.,Eid M., Gao M.Y., Shirley R.B., Stoffel M.T., Kumar M.V., MohnenD.: Pectin induces apoptosis in human prostate cancer cells: correlationof apoptotic function with pectin structure. Glycobiology,2007; 17: 805-819
  Google Scholar
• 21. Jakobsdottir G., Jädert C., Holm L., Nyman M.E.: Propionic andbutyric acids, formed in the caecum of rats fed highly fermentabledietary fibre, are reflected in portal and aortic serum. Br. J. Nutr.,2013; 110: 1565-1572
  Google Scholar
• 22. Jenkins D.J., Leeds A.R., Gassull M.A., Cochet B., Alberti G.M.: Decreasein postprandial insulin and glucose concentrations by guarand pectin. Ann. Intern. Med., 1977; 86: 20-23
  Google Scholar
• 23. Khalil A.H.: Quality of French fried potatoes as influenced bycoating with hydrocolloids. Food Chem., 1999; 66: 201-208
  Google Scholar
• 24. Khotimchenko M.Y., Kolenchenko E.A., Khotimchenko Y.S.: Zincbindingactivity of different pectin compounds in aqueous solutions.J. Colloid Interface Sci., 2008; 323: 216-222
  Google Scholar
• 25. Khotimchenko M.Y., Kolenchenko E.A., Khotimchenko Y.S,Khozhaenko E.V., Kovalev V.V.: Cerium binding activity of differentpectin compounds in aqueous solutions. Colloids Surf. B Biointerfaces,2010; 77: 104-110
  Google Scholar
• 26. Kim M.: Highly esterified pectin with low molecular weightenhances intestinal solubility and absorption of ferric iron in rats.Nutr. Res., 1998; 18: 1981-1994
  Google Scholar
• 27. Kim M.: High-methoxyl pectin has greater enhancing effecton glucose uptake in intestinal perfused rats. Nutrition, 2005; 21:372-377
  Google Scholar
• 28. Koseki M., Tsuji K., Nakagawa Y., Kawamura M., Ichikawa T., KazamaM., Kitabatake M., Doi E.: Effects of gum arabic and pectin onthe emulsification, the lipase reaction, and the plasma cholesterollevel in rats. Agric. Biol. Chem., 1989; 53: 3127-3132
  Google Scholar
• 29. Kumar A., Chauhan G.S.: Extraction and characterization ofpectin from apple pomace and its evaluation as lipase (steapsin)inhibitor. Carbohydr. Polym., 2010; 82: 454-459
  Google Scholar
• 30. Liu L., Fishman M.L., Hicks K.B., Kende M.: Interaction of variouspectin formulations with porcine colonic tissues. Biomaterials,2005; 26: 5907-5916
  Google Scholar
• 31. MacKinnon A.C., Liu X., Hadoke P.W., Miller M.R., Newby D.E.,Sethi T.: Inhibition of galectin-3 reduces atherosclerosis in apolipoproteinE-deficient mice. Glycobiology, 2013; 23: 654-663
  Google Scholar
• 32. Malviya R., Srivastrava P., Bansal M., Sharma P.K.: Mango peel pectinas superdisintegrating agents. J. Sci. Industr. Res., 2010; 69: 688-690
  Google Scholar
• 33. Małecki M.T.: Otyłość – insulinooporność- cukrzyca typu 2. Kardiol.Pol., 2006; 64 (Suppl. 6): 561-566
  Google Scholar
• 34. Marounek M., Volek Z., Synytsya A., Copiková J.: Effect of pectinand amidated pectin on cholesterol homeostasis and cecal metabolismin rats fed a high-cholesterol diet. Physiol. Res., 2007; 56: 433-442
  Google Scholar
• 35. Morris V.J., Gromer A., Kirby A.R., Bongaerts R.J., Gunning A.P.:Using AFM and force spectroscopy to determine pectin structureand (bio)functionality. Food Hydrocoll., 2011; 25: 230-237
  Google Scholar
• 36. Nakahara S., Oka N., Raz A.: On the role of galectin-3 in cancerapoptosis. Apoptosis, 2005; 10: 267-275
  Google Scholar
• 37. Nangia-Makker P., Hogan V., Honjo Y., Baccarini S., Tait L., BresalierR., Raz A.: Inhibition of human cancer cell growth and metastasisin nude mice by oral intake of modified citrus pectin. J. Natl. CancerInst., 2002; 94: 1854-1862
  Google Scholar
• 38. Ohkami H., Tazawa K., Yamashita I., Shimizu T., Murai K., KobashiK., Fujimaki M.: Effects of apple pectin on fecal bacterial enzymesin azoxymethane-induced rat colon carcinogenesis. Jpn. J. CancerRes., 1995; 86: 523-529
  Google Scholar
• 39. Olano-Martin E., Gibson G.R., Rastell R.A.: Comparison of the invitro bifidogenic properties of pectins and pectic-oligosaccharides.J. Appl. Microbiol., 2002; 93: 505-511
  Google Scholar
• 40. Olano-Martin E., Rimbach G.H., Gibson G.R., Rastall R.A.: Pectinand pectic-oligosaccharides induce apoptosis in in vitro humancolonic adenocarcinoma cells. Anticancer Res., 2003; 23: 341-346
  Google Scholar
• 41. Olano-Martin E., Williams M.R., Gibson G.R., Rastall R.A.: Pectinsand pectic-oligosaccharides inhibit Escherichia coli O157:H7 Shigatoxin as directed towards the human colonic cell line HT29. FEMSMicrobiol. Lett., 2003; 218: 101-105
  Google Scholar
• 42. Paulsen B.S., Barsett H.: Bioactive pectic polysaccharides. Adv.Polym. Sci., 2005; 186: 69-101
  Google Scholar
• 43. Pienta K., Naik H., Akhtar A., Yamazaki K., Replogle T.S., LehrJ., Donat T.L., Tait L., Hogan V., Raz A.: Inhibition of spontaneousmetastasis in a rat prostate cancer model by oral administration ofmodified citrus pectin. J. Natl. Cancer Inst., 1995; 87: 348-353
  Google Scholar
• 44. Pilnik W.: Pectin a many splendoured thing. W: Gums and stabilizersfor food industry, Phillips G.O., Williams P.A., Wedlock D.J.(red.), Oxford University Press, 1990, 313-321
  Google Scholar
• 45. Platt D., Raz A.: Modulation of the lung colonization of B16-F1melanoma cells by citrus pectin. J. Natl. Cancer Inst., 1992; 84: 438-442
  Google Scholar
• 46. Ramachandran C., Wilk B.J., Hotchkiss A., Chau H., Eliaz I., MelnickS.J.: Activation of human T-helper/inducer cell, T-cytotoxiccell, B-cell, and natural killer (NK)-cells and induction of naturalkiller cell activity against K562 chronic myeloid leukemia cells withmodified citrus pectin. BMC Complement. Altern. Med., 2011; 11: 59
  Google Scholar
• 47. Ramakrishna B.S., Mathan V.I.: Colonic dysfunction in acute diarrhoea:the role of luminal short chain fatty acids. Gut, 1993; 34: 1215-1218
  Google Scholar
• 48. Renard C.M., Jarvis M.: Acetylation and methylation of homogalacturonans2: effect on ion-binding properties and conformations.Carbohydr. Polym., 1999; 39: 209-216
  Google Scholar
• 49. Ryssel H., Kloeters O., Germann G., Schäfer T., Wiedemann G.,Oehlbauer M.: The antimicrobial effect of acetic acid – an alternativeto common local antiseptics? Burns, 2009; 35: 695-700
  Google Scholar
• 50. Saito D., Nakaji S., Fukuda S., Shimoyama T., Sakamoto J., SugawaraK.: Comparison of the amount of pectin in the human terminalileum with the amount of orally administered pectin. Nutrition,2005; 21: 914-919
  Google Scholar
• 51. Salman H., Bergman M., Djaldetti M., Orlin J., Bessler H.: Citruspectin affects cytokine production by human peripheral bloodmononuclear cells. Biomed. Pharmacother., 2008; 62: 579-582
  Google Scholar
• 52. Sánchez D., Muguerza B., Moulay L., Hernández R., Miguel M.,Aleixandre A.: Highly methoxylated pectin improves insulin resistanceand other cardiometabolic risk factors in Zucker fatty rats. J.Agric. Food Chem., 2008; 56: 3574-3581
  Google Scholar
• 53. Schwab U., Louheranta A., Törrönen A., Uusitupa M.: Impact of sugarbeet pectin and polydextrose on fasting and postprandial glycemia andfasting concentrations of serum total and lipoprotein lipids in middleagedsubjects with abnormal glucose metabolism. Eur. J. Clin. Nutr., 2006;60: 1073-1080
  Google Scholar
• 54. Serguschenko I., Kolenchenko E., Khotimchenko M.: Low esterifiedpectin accelerates removal of lead ions in rats. Nutr. Res., 2007; 27: 633-639
  Google Scholar
• 55. Sergushchenko I.S., Kovalev V.V., Bednyak V.E., KhotimchenkoY.S.: A comparative evaluation of the metal-binding activity of lowesterifiedpectin from the seagrass Zostera marina and other sorbents.Russ. J. Mar. Biol., 2004; 30: 70-72
  Google Scholar
• 56. Sgrebneva M.N., Anisimov A.P., Khasina E.I.: Influence of pectinfrom the seagrass Zostera marina on the content of DNA and RNA in rathepatocytes contaminated by lead. Russ. J. Mar. Biol., 2002; 28: 352-354
  Google Scholar
• 57. Sriamornsak P., Wattanakorn N., Takeuchi H.: Study on the mucoadhesionmechanism of pectin by atomic force microscopy andmucin-particle method. Carbohydr. Polym., 2010; 79: 54-59
  Google Scholar
• 58. Sudheesh S., Vijayalakshmi N.R.: Lipid-lowering action of pectinfrom Cucumis sativus. Food Chem., 1999; 67: 281-286
  Google Scholar
• 59. Tahiri M., Pellerin P., Tressol J.C., Doco T., Pépin D., Rayssiguier Y.,Coudray C.: The rhamnogalacturonan-II dimer decreases intestinal absorptionand tissue accumulation of lead in rats. J. Nutr.,2000; 130: 249-253
  Google Scholar
• 60. Vayssade M., Sengkhamparn N., Verhoef R., Delaigue C., GoundiamO., Vigneron P., Voragen A.G., Schols H.A., Nagel M.D.: Antiproliferativeand proapoptotic actions of okra pectin on B16F10melanoma cells. Phytother. Res., 2010; 24: 982-989
  Google Scholar
• 61. Vigne J.L., Lairon D., Borel P., Portugal H., Pauli A.M., HautonJ.C., Lafont H.: Effect of pectin, wheat bran and cellulose on serumlipids and lipoproteins in rats fed on a low- or high-fat diet. Br. J.Nutr., 1987; 58: 405-413
  Google Scholar
• 62. Voragen A.G., Coenen G.J., Verhoef R.P., Schols H.A.: Pectin, a versatilepolysaccharide present in plant cell walls. Struct. Chem., 2009; 20: 263-275
  Google Scholar
• 63. Wang Y., Nangia-Makker P., Balan V., Hogan V., Raz A.: Calpainactivation through galectin-3 inhibition sensitizes prostate cancercells to cisplatin treatment. Cell Death Dis., 2010; 1: e101
  Google Scholar
• 64. Wikiera A., Mika M.: Budowa i właściwości pektyn. Postępy Biochem.,2013; 59: 89-94
  Google Scholar
• 65. Willats W.G., Knox J.P., Mikkelsen J.D.: Pectin: new insights into an oldpolymer are starting to gel. Trends Food Sci. Technol., 2006; 17: 97-104
  Google Scholar
• 66. Younes H., Demigné C., Rémésy C.: Acidic fermentation in thecaecum increases absorption of calcium and magnesium in the largeintestine of the rat. Br. J. Nutr., 1996; 75: 301-314
  Google Scholar
• 67. Zhao Q., Xie B., Yan J., Zhao F., Xiao J., Yao L., Zhao B., Huang Y.: Invitro antioxidant and antitumor activities of polysaccharides extractedfrom Asparagus officinalis. Carbohydr. Polym., 2012; 87: 392-396
  Google Scholar
• 68. Zhao Z.Y., Liang L., Fan X., Yu Z., Hotchkiss A.T., Wilk B.J., EliazI.: The role of modified citrus pectin as an effective chelator of leadin children hospitalized with toxic lead levels. Altern. Ther. HealthMed., 2008; 14: 34-38
  Google Scholar
• 69. Żółtaszek R., Hanausek M., Kiliańska Z.M., Walaszek Z.: Biologicznarola kwasu D-glukarowego i jego pochodnych: potencjalne zastosowaniew medycynie. Postępy Hig. Med. Dośw., 2008; 62: 451-462
  Google Scholar

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