COMMENTARY ON THE LAW
Therapeutic potential of secondary metabolites produced in the hairy roots cultures
Tomasz Kowalczyk 1 , Marta Łucka 1 , Janusz Szemraj 2 , Tomasz Sakowicz 31. Katedra Genetyki Ogólnej, Biologii Molekularnej i Biotechnologii Roślin, Wydział Biologii i Ochrony Środowiska, Uniwersytet Łódzki, Łódź
2. Zakład Biochemii Medycznej Uniwersytet Medyczny, Łódź
3. Katedra Genetyki Ogólnej, Biologii Molekularnej i Biotechnologii Roślin, Wydział Biologii i Ochrony Środowiska, Uniwersytet Łódzki, Łódź
Published: 2015-05-04
GICID: 01.3001.0009.6530
Available language versions: en pl
Issue: Postepy Hig Med Dosw 2015; 69 : 549-561
Abstract
Plants have always been a source of many valuable substances for humans. Growing advancement of methods of modern biotechnology, combined with genetic engineering techniques, gradually increase the variety of compounds obtained, the number of plant species used and the production efficiency. Consequently, there is an undebatable interest in biotechnological production of such compounds, especially those pharmacologically active, that can be used in treatment of neoplastic, viral, and many other types of diseases. Most of these compounds represent a diverse group of secondary metabolites. One of the effective ways of obtaining such molecules is the utilization of hairy roots cultures. The advantages of such systems make them an attractive method of obtaining important plant-derived compounds, creating an interesting alternative to other methods, including the cell suspension cultures or expensive chemical syntheses.
References
- 1. Aditya N.P., Vathsala P.G., Vieira V., Murthy R.S., Souto E.B.: Advancesin nanomedicines for malaria treatment. Adv. Colloid InterfaceSci., 2013; 201-202: 1-17
Google Scholar - 1. Aditya N.P., Vathsala P.G., Vieira V., Murthy R.S., Souto E.B.: Advancesin nanomedicines for malaria treatment. Adv. Colloid InterfaceSci., 2013; 201-202: 1-17
Google Scholar - 2. Agata K., Kusiak J., Stępień B., Bergier K., Kuźniak E.: Bioaktywnemetabolity wtórne roślin z rodzaju Physalis. Postępy Hig. Med.Dośw., 2010; 64: 665-673
Google Scholar - 2. Agata K., Kusiak J., Stępień B., Bergier K., Kuźniak E.: Bioaktywnemetabolity wtórne roślin z rodzaju Physalis. Postępy Hig. Med.Dośw., 2010; 64: 665-673
Google Scholar - 3. Aharoni A., Jongsma M.A., Kim T.Y., Ri M.B., Giri A.P., VerstappenF.W., Schwab W., Bouwmeester H.J.: Metabolic engineering ofterpenoid biosynthesis in plants. Phytochem. Rev., 2006; 5: 49-58
Google Scholar - 3. Aharoni A., Jongsma M.A., Kim T.Y., Ri M.B., Giri A.P., VerstappenF.W., Schwab W., Bouwmeester H.J.: Metabolic engineering ofterpenoid biosynthesis in plants. Phytochem. Rev., 2006; 5: 49-58
Google Scholar - 4. Alkuraishy H.M., Alwindy S.: Beneficial effects of silymarin onlipid profile in hyperlipidemic patients: placebo controlled clinicaltrial. WebmedCentral Pharmacology, 2012; 3: WMC002966
Google Scholar - 4. Alkuraishy H.M., Alwindy S.: Beneficial effects of silymarin onlipid profile in hyperlipidemic patients: placebo controlled clinicaltrial. WebmedCentral Pharmacology, 2012; 3: WMC002966
Google Scholar - 5. Angelova Z., Georgiev S., Ross W.: Elicitation of plants. Biotechnol.Biotec. Equip., 2006; 20: 72-83
Google Scholar - 5. Angelova Z., Georgiev S., Ross W.: Elicitation of plants. Biotechnol.Biotec. Equip., 2006; 20: 72-83
Google Scholar - 6. Ayora-Talavera T., Chappell J., Lozoya-Gloria E., Loyola-VargasV.M.: Overexpression in Catharanthus roseus hairy roots of a truncatedhamster 3-hydroxy-3-methylglutaryl-CoA reductase gene. Appl.Biochem. Biotechnol., 2002; 97: 135-145
Google Scholar - 6. Ayora-Talavera T., Chappell J., Lozoya-Gloria E., Loyola-VargasV.M.: Overexpression in Catharanthus roseus hairy roots of a truncatedhamster 3-hydroxy-3-methylglutaryl-CoA reductase gene. Appl.Biochem. Biotechnol., 2002; 97: 135-145
Google Scholar - 7. Bhalla R., Narasimhan K., Swarup S.: Metabolomics and its rolein understanding cellular responses in plants. Plant Cell Rep., 2005;24: 562-571
Google Scholar - 7. Bhalla R., Narasimhan K., Swarup S.: Metabolomics and its rolein understanding cellular responses in plants. Plant Cell Rep., 2005;24: 562-571
Google Scholar - 8. Blande J.D., Li T., Holopainen J.K.: Air pollution impedes plant-to–plant communication, but what is the signal? Plant Signal. Behav.,2011; 6: 1016-1018
Google Scholar - 8. Blande J.D., Li T., Holopainen J.K.: Air pollution impedes plant-to–plant communication, but what is the signal? Plant Signal. Behav.,2011; 6: 1016-1018
Google Scholar - 9. Bylka W., Znajdek-Awiżeń P., Studzińska-Sroka E., Dańczak-PazdrowskaA., Brzezińska M.: Centella asiatica in dermatology: an overview.Phytother. Res., 2014; 28: 1117-1124
Google Scholar - 9. Bylka W., Znajdek-Awiżeń P., Studzińska-Sroka E., Dańczak-PazdrowskaA., Brzezińska M.: Centella asiatica in dermatology: an overview.Phytother. Res., 2014; 28: 1117-1124
Google Scholar - 10. Calić-Dragosavac D., Zdravković-Korać S., Savikin-Fodulović K.,Radojević L., Vinterhalter B.: Determination of escin content in androgenicembryos and hairy root culture of Aesculus hippocastanum.Pharm. Biol., 2010: 48: 563-567
Google Scholar - 10. Calić-Dragosavac D., Zdravković-Korać S., Savikin-Fodulović K.,Radojević L., Vinterhalter B.: Determination of escin content in androgenicembryos and hairy root culture of Aesculus hippocastanum.Pharm. Biol., 2010: 48: 563-567
Google Scholar - 11. Cardillo A. B., Otalvaro A.A., Busto V.D., Rodriguez Talou J., VelasquezL.M., Giulietti A.M.: Scopolamine, anisodamine and hyoscyamineproduction by Brugmansia candida hairy root cultures in bioreactors.Process Biochem., 2010; 45: 1577-1581
Google Scholar - 11. Cardillo A. B., Otalvaro A.A., Busto V.D., Rodriguez Talou J., VelasquezL.M., Giulietti A.M.: Scopolamine, anisodamine and hyoscyamineproduction by Brugmansia candida hairy root cultures in bioreactors.Process Biochem., 2010; 45: 1577-1581
Google Scholar - 12. Chandra S., Chandra R.: Engineering secondary metabolite productionin hairy roots. Phytochem. Rev., 2011; 10: 371-395
Google Scholar - 12. Chandra S., Chandra R.: Engineering secondary metabolite productionin hairy roots. Phytochem. Rev., 2011; 10: 371-395
Google Scholar - 13. Chandra S., Chandra R., Sharma H.P., Jha S.: Agrobacterium rhizogenesmediated genetic transformation of Spilanthes paniculata (DC.)Jansen. Int. J. Plant Sci., 2008; 3: 471-476
Google Scholar - 13. Chandra S., Chandra R., Sharma H.P., Jha S.: Agrobacterium rhizogenesmediated genetic transformation of Spilanthes paniculata (DC.)Jansen. Int. J. Plant Sci., 2008; 3: 471-476
Google Scholar - 14. Chaturvedi D., Goswami A., Saikia P.P., Barua N.C., Rao P.G.: Artemisininand its derivatives: a novel class of anti-malarial and anti–cancer agents. Chem. Soc. Rev., 2010; 39: 435-454
Google Scholar - 14. Chaturvedi D., Goswami A., Saikia P.P., Barua N.C., Rao P.G.: Artemisininand its derivatives: a novel class of anti-malarial and anti–cancer agents. Chem. Soc. Rev., 2010; 39: 435-454
Google Scholar - 15. Cheng A.X., Lou Y.G., Mao Y.B., Lu S., Wang L.J., Chen X.Y.: Plantterpenoids: biosynthesis and ecological functions. J. Integr. PlantBiol., 2007; 49: 179-186
Google Scholar - 15. Cheng A.X., Lou Y.G., Mao Y.B., Lu S., Wang L.J., Chen X.Y.: Plantterpenoids: biosynthesis and ecological functions. J. Integr. PlantBiol., 2007; 49: 179-186
Google Scholar - 16. Choi K.T.: Botanical characteristics, pharmacological effectsand medicinal components of Korean Panax ginseng C.A. Meyer. ActaPharmacol. Sin., 2008; 29: 1109-1118
Google Scholar - 16. Choi K.T.: Botanical characteristics, pharmacological effectsand medicinal components of Korean Panax ginseng C.A. Meyer. ActaPharmacol. Sin., 2008; 29: 1109-1118
Google Scholar - 17. Dare A.P., Schaffer R.J., Lin-Wang K., Allan A.C., Hellens R.P.:Identification of a cis-regulatory element by transient analysis ofco-ordinately regulated genes. Plant Methods, 2008; 4: 17-27
Google Scholar - 17. Dare A.P., Schaffer R.J., Lin-Wang K., Allan A.C., Hellens R.P.:Identification of a cis-regulatory element by transient analysis ofco-ordinately regulated genes. Plant Methods, 2008; 4: 17-27
Google Scholar - 18. Drozak J., Bryła J.: Dopamine: not just a neurotransmitter. PostępyHig. Med. Dośw., 2005; 59: 405-420
Google Scholar - 18. Drozak J., Bryła J.: Dopamine: not just a neurotransmitter. PostępyHig. Med. Dośw., 2005; 59: 405-420
Google Scholar - 19. Eibl R., Eibl D.: Design of bioreactors suitable for plant cell andtissue cultures. Phytochem. Rev., 2008; 7: 593-598
Google Scholar - 19. Eibl R., Eibl D.: Design of bioreactors suitable for plant cell andtissue cultures. Phytochem. Rev., 2008; 7: 593-598
Google Scholar - 20. Evans W.C.: Alkaloids. W: Pharmacognosy, red.: Evans W.C., TreaseG.E. Saunders Ltd, Philadelphia 2009, 353-415
Google Scholar - 20. Evans W.C.: Alkaloids. W: Pharmacognosy, red.: Evans W.C., TreaseG.E. Saunders Ltd, Philadelphia 2009, 353-415
Google Scholar - 21. Frankfater C.R., Dowd M.K., Triplett B.A.: Effect of elicitors onthe production of gossypol and methylated gossypol in cotton hairyroots. Plant Cell Tiss. Organ Cult., 2009; 98: 341-349
Google Scholar - 21. Frankfater C.R., Dowd M.K., Triplett B.A.: Effect of elicitors onthe production of gossypol and methylated gossypol in cotton hairyroots. Plant Cell Tiss. Organ Cult., 2009; 98: 341-349
Google Scholar - 22. Gaume A., Komarnytsky S., Borisjuk N., Raskin I.: Rhizosecretionof recombinant proteins from plant hairy roots. Plant Cell Rep.,2003; 21: 1188-1193
Google Scholar - 22. Gaume A., Komarnytsky S., Borisjuk N., Raskin I.: Rhizosecretionof recombinant proteins from plant hairy roots. Plant Cell Rep.,2003; 21: 1188-1193
Google Scholar - 23. Georgiev M.I., Agostini E., Ludwig-Muller J., Xu J.: Geneticallytransformed roots: from plant disease to biotechnological resource.Trends Biotechnol., 2012; 30, 528-537
Google Scholar - 23. Georgiev M.I., Agostini E., Ludwig-Muller J., Xu J.: Geneticallytransformed roots: from plant disease to biotechnological resource.Trends Biotechnol., 2012; 30, 528-537
Google Scholar - 24. Georgiev M.I., Ludwig-Muller J., Bley T.: Hairy root culture: copyingnature in new bioprocesses. W: Medicinal Plant Biotechnology,red.: Arora R., CAB International, 2010, 156-175
Google Scholar - 24. Georgiev M.I., Ludwig-Muller J., Bley T.: Hairy root culture: copyingnature in new bioprocesses. W: Medicinal Plant Biotechnology,red.: Arora R., CAB International, 2010, 156-175
Google Scholar - 25. Goel M.K., Mehrotra S., Kukreja A.K.: Elicitor-induced cellularand molecular events are responsible for productivity enhancementin hairy root cultures: an insight study. Appl. Biochem. Biotechnol.,2011: 165: 1342-1355
Google Scholar - 25. Goel M.K., Mehrotra S., Kukreja A.K.: Elicitor-induced cellularand molecular events are responsible for productivity enhancementin hairy root cultures: an insight study. Appl. Biochem. Biotechnol.,2011: 165: 1342-1355
Google Scholar - 26. Gohil K.J., Patel J.A., Gajjar A.K.: Pharmacological review onCentella asiatica: a potential herbal cure-all. Indian J. Pharm. Sci.,2010; 72: 546-556
Google Scholar - 26. Gohil K.J., Patel J.A., Gajjar A.K.: Pharmacological review onCentella asiatica: a potential herbal cure-all. Indian J. Pharm. Sci.,2010; 72: 546-556
Google Scholar - 27. Gomez-Galera S., Pelacho A.M., Gene A., Capell T., Christou P.:The genetic manipulation of medicinal and aromatic plants. PlantCell Rep., 2007; 26: 1689-1715
Google Scholar - 27. Gomez-Galera S., Pelacho A.M., Gene A., Capell T., Christou P.:The genetic manipulation of medicinal and aromatic plants. PlantCell Rep., 2007; 26: 1689-1715
Google Scholar - 28. Guillon S., Tremouillaux-Guiller J., Pati P.K., Rideau M., GantetP.: Hairy root research: recent scenario and exciting prospects. Curr.Opin. Plant Biol., 2006; 9: 341-346
Google Scholar - 28. Guillon S., Tremouillaux-Guiller J., Pati P.K., Rideau M., GantetP.: Hairy root research: recent scenario and exciting prospects. Curr.Opin. Plant Biol., 2006; 9: 341-346
Google Scholar - 29. Guirimand G., Courdavault V., St-Pierre B., Burlat V.: Biosynthesisand regulation of alkaloids. W: Plant Developmental Biology- Biotechnological Perspectives, red.: Pua E.C., Davey M.R. Springer,Berlin Heidelberg 2010, 139-160
Google Scholar - 29. Guirimand G., Courdavault V., St-Pierre B., Burlat V.: Biosynthesisand regulation of alkaloids. W: Plant Developmental Biology- Biotechnological Perspectives, red.: Pua E.C., Davey M.R. Springer,Berlin Heidelberg 2010, 139-160
Google Scholar - 30. Guo R., Wei P., Liu W.: Combined antioxidant effects of rutinand vitamin C in Triton X-100 micelles. J. Pharm. Biomed. Anal.,2007; 43: 1580-1586
Google Scholar - 30. Guo R., Wei P., Liu W.: Combined antioxidant effects of rutinand vitamin C in Triton X-100 micelles. J. Pharm. Biomed. Anal.,2007; 43: 1580-1586
Google Scholar - 31. Hagel J.M., Macleod B.P., Facchini P.J.: Opium poppy. W: Biotechnologyin Agriculture and Forestry. Transgenic Crops VI, red.: PuaE.C., Davey M.R. Springer, Berlin Heidelberg 2007, 169-187
Google Scholar - 31. Hagel J.M., Macleod B.P., Facchini P.J.: Opium poppy. W: Biotechnologyin Agriculture and Forestry. Transgenic Crops VI, red.: PuaE.C., Davey M.R. Springer, Berlin Heidelberg 2007, 169-187
Google Scholar - 32. Hendrawati O., Woerdenbag H.J., Hille J., Kayser O.: Metabolicengineering strategies for the optimization of medicinal and aromaticplants: realities and expectations. Zeitschrift für Arznei- &Gewürzpflanzen, 2010; 15: 111-126
Google Scholar - 32. Hendrawati O., Woerdenbag H.J., Hille J., Kayser O.: Metabolicengineering strategies for the optimization of medicinal and aromaticplants: realities and expectations. Zeitschrift für Arznei- &Gewürzpflanzen, 2010; 15: 111-126
Google Scholar - 33. Hsu S.C., Chung J.G.: Anticancer potential of emodin. BioMedicine,2012; 2: 108-116
Google Scholar - 33. Hsu S.C., Chung J.G.: Anticancer potential of emodin. BioMedicine,2012; 2: 108-116
Google Scholar - 34. Hunyadi A., Martins A., Hsieh T.J., Seres A., Zupko I.: Chlorogenicacid and rutin play a major role in the in vivo anti-diabeticactivity of Morus alba leaf extract on type II diabetic rats. PLoS One,2012; 7: e50619
Google Scholar - 34. Hunyadi A., Martins A., Hsieh T.J., Seres A., Zupko I.: Chlorogenicacid and rutin play a major role in the in vivo anti-diabeticactivity of Morus alba leaf extract on type II diabetic rats. PLoS One,2012; 7: e50619
Google Scholar - 35. Hyam S.R., Jang S.E., Jeong J.J., Joh E.H., Han M.J., Kim D.H.: Echinocysticacid, a metabolite of lancemaside A, inhibits TNBS-inducedcolitis in mice. Int. Immunopharmacol., 2013; 15: 433-441
Google Scholar - 35. Hyam S.R., Jang S.E., Jeong J.J., Joh E.H., Han M.J., Kim D.H.: Echinocysticacid, a metabolite of lancemaside A, inhibits TNBS-inducedcolitis in mice. Int. Immunopharmacol., 2013; 15: 433-441
Google Scholar - 36. Ionkova I., Momekov G., Proksch P.: Effects of cycloartane saponinsfrom hairy roots of Astragalus membranaceus Bge., on humantumor cell targets. Fitoterapia, 2010; 81: 447-451
Google Scholar - 36. Ionkova I., Momekov G., Proksch P.: Effects of cycloartane saponinsfrom hairy roots of Astragalus membranaceus Bge., on humantumor cell targets. Fitoterapia, 2010; 81: 447-451
Google Scholar - 37. Jang J., Jeong S.J., Kwon H.Y., Jung J.H., Sohn E.J, Lee H.J., KimJ.H., Kim S.H., Kim J.H., Kim S.H.: Decursin and doxorubicin are insynergy for the induction of apoptosis via STAT3 and/or mTOR pathwaysin human multiple myeloma cells. Evid. Based Complement.Alternat. Med., 2013; 2013: 1-13
Google Scholar - 37. Jang J., Jeong S.J., Kwon H.Y., Jung J.H., Sohn E.J, Lee H.J., KimJ.H., Kim S.H., Kim J.H., Kim S.H.: Decursin and doxorubicin are insynergy for the induction of apoptosis via STAT3 and/or mTOR pathwaysin human multiple myeloma cells. Evid. Based Complement.Alternat. Med., 2013; 2013: 1-13
Google Scholar - 38. Jelassi B., Anchelin M., Chamouton J., Cayuela M.L., Clarysse L.,Li J., Goré J., Jiang L.H., Roger S.: Anthraquinone emodin inhibitshuman cancer cell invasiveness by antagonizing P2X7 receptors.Carcinogenesis, 2013; 34: 1487-1496
Google Scholar - 38. Jelassi B., Anchelin M., Chamouton J., Cayuela M.L., Clarysse L.,Li J., Goré J., Jiang L.H., Roger S.: Anthraquinone emodin inhibitshuman cancer cell invasiveness by antagonizing P2X7 receptors.Carcinogenesis, 2013; 34: 1487-1496
Google Scholar - 39. Jeremicz Z., Luczkiewicz M., Kokotkiewicz A., Królicka A., SowińskiP.: Production of tropane alkaloids in Hyoscyamus niger (blackhenbane) hairy roots grown in bubble-column and spray bioreactors.Biotechnol. Lett., 2014; 36: 843-853
Google Scholar - 39. Jeremicz Z., Luczkiewicz M., Kokotkiewicz A., Królicka A., SowińskiP.: Production of tropane alkaloids in Hyoscyamus niger (blackhenbane) hairy roots grown in bubble-column and spray bioreactors.Biotechnol. Lett., 2014; 36: 843-853
Google Scholar - 40. Kang Y.M., Park D.J., Min J.Y., Song H.J., Jeong M.J., Kim Y.D., KangS.M., Karigar C.S., Choi M.S.: Enhanced production of tropane alkaloidsin transgenic Scopolia parviflora hairy root cultures over-expressingputrescine N-methyl transferase (PMT) and hyoscyamine-6β-hydroxylase (H6H). In Vitro Cell. Dev. Biol. − Plant, 2011; 47: 516-524
Google Scholar - 40. Kang Y.M., Park D.J., Min J.Y., Song H.J., Jeong M.J., Kim Y.D., KangS.M., Karigar C.S., Choi M.S.: Enhanced production of tropane alkaloidsin transgenic Scopolia parviflora hairy root cultures over-expressingputrescine N-methyl transferase (PMT) and hyoscyamine-6β-hydroxylase (H6H). In Vitro Cell. Dev. Biol. − Plant, 2011; 47: 516-524
Google Scholar - 41. Kawiak A., Zawacka-Pankau J., Lojkowska E.: Plumbagin inducesapoptosis in Her2-overexpressing breast cancer cells through themitochondrial-mediated pathway. J. Nat. Prod., 2012; 75: 747-751
Google Scholar - 41. Kawiak A., Zawacka-Pankau J., Lojkowska E.: Plumbagin inducesapoptosis in Her2-overexpressing breast cancer cells through themitochondrial-mediated pathway. J. Nat. Prod., 2012; 75: 747-751
Google Scholar - 42. Khalili M., Hasanloo T., Kazemi Tabar S.K., Rahnama H.: Influenceof exogenous salicylic acid on flavonolignans and lipoxygenaseactivity in the hairy root cultures of Silybum marianum. Cell Biol.Int., 2009; 33: 988-994
Google Scholar - 42. Khalili M., Hasanloo T., Kazemi Tabar S.K., Rahnama H.: Influenceof exogenous salicylic acid on flavonolignans and lipoxygenaseactivity in the hairy root cultures of Silybum marianum. Cell Biol.Int., 2009; 33: 988-994
Google Scholar - 43. Khalili M., Hasanloo T., Tabar S.K.: Ag+ enhanced silymarin productionin hairy root cultures of Silybum marianum (L.) Gaertn. POJ,2010; 3: 109-114
Google Scholar - 43. Khalili M., Hasanloo T., Tabar S.K.: Ag+ enhanced silymarin productionin hairy root cultures of Silybum marianum (L.) Gaertn. POJ,2010; 3: 109-114
Google Scholar - 44. Kim J.A., Baek K.H., Son Y.M., Son S.H., Shin H.: Hairy root culturesof Taxus cuspidata for enhanced production of paclitaxel. J.Korean Soc. Appl. Biol. Chem., 2009; 52: 144-150
Google Scholar - 44. Kim J.A., Baek K.H., Son Y.M., Son S.H., Shin H.: Hairy root culturesof Taxus cuspidata for enhanced production of paclitaxel. J.Korean Soc. Appl. Biol. Chem., 2009; 52: 144-150
Google Scholar - 45. Kim J.A., Kim Y.S., Choi Y.E.: Triterpenoid production and phenotypicchanges in hairy roots of Codonopsis lanceolata and the plantsregenerated from them. Plant Biotechnol. Rep., 2011; 5: 255-263
Google Scholar - 45. Kim J.A., Kim Y.S., Choi Y.E.: Triterpenoid production and phenotypicchanges in hairy roots of Codonopsis lanceolata and the plantsregenerated from them. Plant Biotechnol. Rep., 2011; 5: 255-263
Google Scholar - 46. Kim O.T., Bang K.H., Kim Y.C., Hyun D.Y., Kim M.Y., Cha S.W.:Upregulation of ginsenoside and gene expression related to triterpenebiosynthesis in ginseng hairy root cultures elicited by methyljasmonate. Plant Cell Tissue Organ Cult., 2009; 98: 25-33
Google Scholar - 46. Kim O.T., Bang K.H., Kim Y.C., Hyun D.Y., Kim M.Y., Cha S.W.:Upregulation of ginsenoside and gene expression related to triterpenebiosynthesis in ginseng hairy root cultures elicited by methyljasmonate. Plant Cell Tissue Organ Cult., 2009; 98: 25-33
Google Scholar - 47. Kim O.T., Bang K.H., Shin Y.S., Lee M.J., Jung S.J., Hyun D.Y.,Kim Y.C., Senong N.S., Cha S.W., Hwang B.: Enhanced production ofasiaticoside from hairy root cultures of Centella asiatica (L.) Urbanelicited by methyl jasmonate. Plant Cell Rep., 2007; 26: 1914-1919
Google Scholar - 47. Kim O.T., Bang K.H., Shin Y.S., Lee M.J., Jung S.J., Hyun D.Y.,Kim Y.C., Senong N.S., Cha S.W., Hwang B.: Enhanced production ofasiaticoside from hairy root cultures of Centella asiatica (L.) Urbanelicited by methyl jasmonate. Plant Cell Rep., 2007; 26: 1914-1919
Google Scholar - 48. Kim W.J., Lee S.J., Choi Y.D., Moon S.K.: Decursin inhibits growthof human bladder and colon cancer cells via apoptosis, G1-phase cellcycle arrest and extracellular signal-regulated kinase activation. Int.J. Mol. Med., 2010; 25: 635-641
Google Scholar - 48. Kim W.J., Lee S.J., Choi Y.D., Moon S.K.: Decursin inhibits growthof human bladder and colon cancer cells via apoptosis, G1-phase cellcycle arrest and extracellular signal-regulated kinase activation. Int.J. Mol. Med., 2010; 25: 635-641
Google Scholar - 49. Ko S., Liu J.R., Yamakawa T., Matsumoto Y.: Expression of theprotective antigen (SpaA) in transgenic hairy roots of tobacco. PlantMol. Biol. Rep., 2006; 24: 251a-251g
Google Scholar - 49. Ko S., Liu J.R., Yamakawa T., Matsumoto Y.: Expression of theprotective antigen (SpaA) in transgenic hairy roots of tobacco. PlantMol. Biol. Rep., 2006; 24: 251a-251g
Google Scholar - 50. Kolewe M.E., Gaurav V., Roberts S.C.: Pharmaceutically activenatural product synthesis and supply via plant cell culture technology.Mol. Pharm., 2008; 5: 243-256
Google Scholar - 50. Kolewe M.E., Gaurav V., Roberts S.C.: Pharmaceutically activenatural product synthesis and supply via plant cell culture technology.Mol. Pharm., 2008; 5: 243-256
Google Scholar - 51. Komarnytsky S., Borisjuk N., Yakoby N., Garvey A., Raskin I.:Cosecretion of protease inhibitor stabilizes antibodies produced byplant roots. Plant Physiol., 2006; 141: 1185-1193
Google Scholar - 51. Komarnytsky S., Borisjuk N., Yakoby N., Garvey A., Raskin I.:Cosecretion of protease inhibitor stabilizes antibodies produced byplant roots. Plant Physiol., 2006; 141: 1185-1193
Google Scholar - 52. Kumar N., Chornokur G.: Molecular targeted therapies usingbotanicals for prostate cancer chemoprevention. Transl. Med., 2012;Suppl. 2: 005
Google Scholar - 52. Kumar N., Chornokur G.: Molecular targeted therapies usingbotanicals for prostate cancer chemoprevention. Transl. Med., 2012;Suppl. 2: 005
Google Scholar - 53. Kuźma L., Wysokińska H., Różalski M., Budzyńska A., Więckowska-SzakielM., Sadowska B., Paszkiewicz M., Kisiel W., Różalska B.:Antimicrobial and anti-biofilm properties of new taxodione derivativefrom hairy roots of Salvia austriaca. Phytomedicine, 2012;19: 1285-1287
Google Scholar - 53. Kuźma L., Wysokińska H., Różalski M., Budzyńska A., Więckowska-SzakielM., Sadowska B., Paszkiewicz M., Kisiel W., Różalska B.:Antimicrobial and anti-biofilm properties of new taxodione derivativefrom hairy roots of Salvia austriaca. Phytomedicine, 2012;19: 1285-1287
Google Scholar - 54. Lavania U.: Genomic and ploidy manipulation for enhanced productionof phyto-pharmaceuticals. Plant Gene Res., 2005; 3: 170-177
Google Scholar - 54. Lavania U.: Genomic and ploidy manipulation for enhanced productionof phyto-pharmaceuticals. Plant Gene Res., 2005; 3: 170-177
Google Scholar - 55. Lee J.H., Yeon J.H., Kim H., Roh W., Chae J., Park H.O., Kim D.M.:The natural anticancer agent plumbagin induces potent cytotoxicityin MCF-7 human breast cancer cells by inhibiting a PI-5 kinasefor ROS generation. PLoS One, 2012; 7: e45023
Google Scholar - 55. Lee J.H., Yeon J.H., Kim H., Roh W., Chae J., Park H.O., Kim D.M.:The natural anticancer agent plumbagin induces potent cytotoxicityin MCF-7 human breast cancer cells by inhibiting a PI-5 kinasefor ROS generation. PLoS One, 2012; 7: e45023
Google Scholar - 56. Lee K.T., Suzuki T., Yamakawa T., Kodama T., Igarashi Y., ShimomuraK.: Production of tropane alkaloids by transformed rootcultures of Atropa belladonna in stirred bioreactors with a stainlesssteel net. Plant Cell Rep., 1999; 18: 567-571
Google Scholar - 56. Lee K.T., Suzuki T., Yamakawa T., Kodama T., Igarashi Y., ShimomuraK.: Production of tropane alkaloids by transformed rootcultures of Atropa belladonna in stirred bioreactors with a stainlesssteel net. Plant Cell Rep., 1999; 18: 567-571
Google Scholar - 57. Lee S.Y., Cho S.J., Park M.H., Kim Y.K., Choi J.E., Park S.U.: Growthand rutin production in hairy root cultures of buckwheat (Fagopyrumesculentum M.). Prep. Biochem. Biotechnol., 2007; 37: 239-246
Google Scholar - 57. Lee S.Y., Cho S.J., Park M.H., Kim Y.K., Choi J.E., Park S.U.: Growthand rutin production in hairy root cultures of buckwheat (Fagopyrumesculentum M.). Prep. Biochem. Biotechnol., 2007; 37: 239-246
Google Scholar - 58. Lee Y.R., Yu D.S., Liang Y.C., Huang K.F., Chou S.J., Chen T.C., LeeC.C., Chen C.L., Chiou S.H., Huang H.S.: New approaches of PARP-1inhibitors in human lung cancer cells and cancer stem-like cells bysome selected anthraquinone-derived small molecules. PLoS One,2013; 8: e56284
Google Scholar - 58. Lee Y.R., Yu D.S., Liang Y.C., Huang K.F., Chou S.J., Chen T.C., LeeC.C., Chen C.L., Chiou S.H., Huang H.S.: New approaches of PARP-1inhibitors in human lung cancer cells and cancer stem-like cells bysome selected anthraquinone-derived small molecules. PLoS One,2013; 8: e56284
Google Scholar - 59. Li F.X., Jin Z.P., Zhao D.X., Cheng L.Q., Fu C.X., Ma F.: Overexpressionof the Saussurea medusa chalcone isomerase gene in S. involucratahairy root cultures enhances their biosynthesis of apigenin.Phytochemistry, 2006; 67: 553-560
Google Scholar - 59. Li F.X., Jin Z.P., Zhao D.X., Cheng L.Q., Fu C.X., Ma F.: Overexpressionof the Saussurea medusa chalcone isomerase gene in S. involucratahairy root cultures enhances their biosynthesis of apigenin.Phytochemistry, 2006; 67: 553-560
Google Scholar - 60. Li Y.C., He S.M., He Z.X., Li M., Yang Y., Pang J.X., Zhang X., ChowK., Zhou Q., Duan W., Zhou Z.W., Yang T., Huang G.H., Liu A., Qiu J.X.,Liu J.P., Zhou S.F.: Plumbagin induces apoptotic and autophagic celldeath through inhibition of the PI3K/Akt/mTOR pathway in humannon-small cell lung cancer cells. Cancer Lett., 2014; 344: 239-259
Google Scholar - 60. Li Y.C., He S.M., He Z.X., Li M., Yang Y., Pang J.X., Zhang X., ChowK., Zhou Q., Duan W., Zhou Z.W., Yang T., Huang G.H., Liu A., Qiu J.X.,Liu J.P., Zhou S.F.: Plumbagin induces apoptotic and autophagic celldeath through inhibition of the PI3K/Akt/mTOR pathway in humannon-small cell lung cancer cells. Cancer Lett., 2014; 344: 239-259
Google Scholar - 61. Lichtenthaler H.K.: The 1-deoxy-d-xylulose-5-phosphate pathwayof isoprenoid biosynthesis in plants. Annu. Rev. Plant Physiol.Plant Mol. Biol., 1999; 50: 47-65
Google Scholar - 61. Lichtenthaler H.K.: The 1-deoxy-d-xylulose-5-phosphate pathwayof isoprenoid biosynthesis in plants. Annu. Rev. Plant Physiol.Plant Mol. Biol., 1999; 50: 47-65
Google Scholar - 62. Lim L.S., Hoeksema L.J., Sherin K., ACPM Prevention PracticeCommittee: Screening for osteoporosis in the adult U.S. population:ACPM position statement on preventive practice. Am. J. Prev.Med., 2009; 36: 366-375
Google Scholar - 62. Lim L.S., Hoeksema L.J., Sherin K., ACPM Prevention PracticeCommittee: Screening for osteoporosis in the adult U.S. population:ACPM position statement on preventive practice. Am. J. Prev.Med., 2009; 36: 366-375
Google Scholar - 63. Liu J., Dolan M.C., Reidy M., Cramer C.L.: Expression of bioactivesingle-chain murine IL-12 in transgenic plants. J. Interferon CytokineRes., 2008; 28: 381-392
Google Scholar - 63. Liu J., Dolan M.C., Reidy M., Cramer C.L.: Expression of bioactivesingle-chain murine IL-12 in transgenic plants. J. Interferon CytokineRes., 2008; 28: 381-392
Google Scholar - 64. Liu Y.X., Shen N.Y., Liu C., Lv Y.: Immunosuppressive effects of emodin:an in vivo and in vitro study. Transplant Proc., 2009; 41: 1837-1839
Google Scholar - 64. Liu Y.X., Shen N.Y., Liu C., Lv Y.: Immunosuppressive effects of emodin:an in vivo and in vitro study. Transplant Proc., 2009; 41: 1837-1839
Google Scholar - 65. Luan J.B., Yao D.M., Zhang T., Walling L.L., Yang M., Wang Y.J.,Liu S.S.: Suppression of terpenoid synthesis in plants by a viruspromotes its mutualism with vectors. Ecol. Lett., 2013; 16: 390-398
Google Scholar - 65. Luan J.B., Yao D.M., Zhang T., Walling L.L., Yang M., Wang Y.J.,Liu S.S.: Suppression of terpenoid synthesis in plants by a viruspromotes its mutualism with vectors. Ecol. Lett., 2013; 16: 390-398
Google Scholar - 66. Mannan A., Shaheen N., Arshad W., Qureshi R.A., Zia M., MirzaB.: Hairy roots induction and artemisinin analysis in Artemisia dubiaand Artemisia indica. Afr. J. Biotechnol., 2008; 7: 3288-3292
Google Scholar - 66. Mannan A., Shaheen N., Arshad W., Qureshi R.A., Zia M., MirzaB.: Hairy roots induction and artemisinin analysis in Artemisia dubiaand Artemisia indica. Afr. J. Biotechnol., 2008; 7: 3288-3292
Google Scholar - 67. Mehrotra S., Kukreja A.K., Khanuja S.P.S., Mishra B.N.: Genetictransformation studies and scale up of hairy root culture of Glycyrrhizaglabra in bioreactor. Electron. J. Biotechn., 2008; 11: 717-728
Google Scholar - 67. Mehrotra S., Kukreja A.K., Khanuja S.P.S., Mishra B.N.: Genetictransformation studies and scale up of hairy root culture of Glycyrrhizaglabra in bioreactor. Electron. J. Biotechn., 2008; 11: 717-728
Google Scholar - 68. Mehrotra S., Rahman L.U., Kukreja A.K.: An extensive case studyof hairy-root cultures for enhanced secondary-metabolite productionthrough metabolic-pathway engineering. Biotechnol. Appl.Biochem., 2010; 56: 161-172
Google Scholar - 68. Mehrotra S., Rahman L.U., Kukreja A.K.: An extensive case studyof hairy-root cultures for enhanced secondary-metabolite productionthrough metabolic-pathway engineering. Biotechnol. Appl.Biochem., 2010; 56: 161-172
Google Scholar - 69. Mishra B.N., Ranjan R.: Growth of hairy-root cultures in variousbioreactors for the production of secondary metabolites. Biotechnol.Appl. Biochem., 2008; 49: 1-10
Google Scholar - 69. Mishra B.N., Ranjan R.: Growth of hairy-root cultures in variousbioreactors for the production of secondary metabolites. Biotechnol.Appl. Biochem., 2008; 49: 1-10
Google Scholar - 70. Moon M.K., Han Y.M., Lee Y.J., Lee L.H., Yang J.H., Kwon B.M.,Kim D.K.: Inhibitory activities of anthraquinones from Rubia akaneon phosphatase regenerating liver-3. Arch. Pharm. Res., 2010; 33:1747-1751
Google Scholar - 70. Moon M.K., Han Y.M., Lee Y.J., Lee L.H., Yang J.H., Kwon B.M.,Kim D.K.: Inhibitory activities of anthraquinones from Rubia akaneon phosphatase regenerating liver-3. Arch. Pharm. Res., 2010; 33:1747-1751
Google Scholar - 71. Moyano E., Jouhikainen K., Tammela P., Palazón J., Cusidó R.M.,Pińol M.T., Teeri T.H., Oksman-Caldentey K.M.: Effect of pmt geneoverexpression on tropane alkaloid production in transformed rootcultures of Datura metel and Hyoscyamus muticus. J. Exp. Bot., 2003;54: 203-211
Google Scholar - 71. Moyano E., Jouhikainen K., Tammela P., Palazón J., Cusidó R.M.,Pińol M.T., Teeri T.H., Oksman-Caldentey K.M.: Effect of pmt geneoverexpression on tropane alkaloid production in transformed rootcultures of Datura metel and Hyoscyamus muticus. J. Exp. Bot., 2003;54: 203-211
Google Scholar - 72. Murthy H.N., Dijkstra C., Anthony P., White D.A., Davey M.R.,Power J.B., Hahn E.J., Paek K.Y.: Establishment of Withania somniferahairy root cultures for the production of withanolide A. J. Integr.Plant Biol., 2008; 50: 975-981
Google Scholar - 72. Murthy H.N., Dijkstra C., Anthony P., White D.A., Davey M.R.,Power J.B., Hahn E.J., Paek K.Y.: Establishment of Withania somniferahairy root cultures for the production of withanolide A. J. Integr.Plant Biol., 2008; 50: 975-981
Google Scholar - 73. Oh C.H., Kang P.S., Kim J.W., Kwon J., Oh S.H.: Water extractsof cultured mountain ginseng stimulate immune cells and inhibitcancer cell proliferation. Food Sci. Biotechnol., 2006; 15: 369-373
Google Scholar - 73. Oh C.H., Kang P.S., Kim J.W., Kwon J., Oh S.H.: Water extractsof cultured mountain ginseng stimulate immune cells and inhibitcancer cell proliferation. Food Sci. Biotechnol., 2006; 15: 369-373
Google Scholar - 74. Oksman-Caldentey K.M., Saito K.: Integrating genomics and metabolomicsfor engineering plant metabolic pathways. Curr. Opin.Biotechnol., 2005; 16: 174-179
Google Scholar - 74. Oksman-Caldentey K.M., Saito K.: Integrating genomics and metabolomicsfor engineering plant metabolic pathways. Curr. Opin.Biotechnol., 2005; 16: 174-179
Google Scholar - 75. Ono N., Tian L.: The multiplicity of hairy root cultures: prolificpossibilities. Plant Sci., 2011; 180: 439-446
Google Scholar - 75. Ono N., Tian L.: The multiplicity of hairy root cultures: prolificpossibilities. Plant Sci., 2011; 180: 439-446
Google Scholar - 76. Park S.U., Facchini P.J.: Agrobacterium rhizogenes-mediated transformationof opium poppy, Papaver somniferum L., and Californiapoppy, Eschscholzia californica Cham., root cultures. J. Exp. Bot., 2000;51: 1005-1016
Google Scholar - 76. Park S.U., Facchini P.J.: Agrobacterium rhizogenes-mediated transformationof opium poppy, Papaver somniferum L., and Californiapoppy, Eschscholzia californica Cham., root cultures. J. Exp. Bot., 2000;51: 1005-1016
Google Scholar - 77. Park S.U., Lee S.Y.: Anthraquinone production by hairy root cultureof Rubia akane Nakai: influence of media and auxin treatment.Sci. Res. Essays, 2009; 4: 690-693
Google Scholar - 77. Park S.U., Lee S.Y.: Anthraquinone production by hairy root cultureof Rubia akane Nakai: influence of media and auxin treatment.Sci. Res. Essays, 2009; 4: 690-693
Google Scholar - 78. Peebles C.A., Hong S.B., Gibson S.I., Shanks J.V., San K.Y.: Effectsof terpenoid precursor feeding on C. roseus hairy roots over-expressingthe alpha or the alpha and beta subunits of anthranilate synthase.Biotechnol. Bioeng., 2006; 93: 534-540
Google Scholar - 78. Peebles C.A., Hong S.B., Gibson S.I., Shanks J.V., San K.Y.: Effectsof terpenoid precursor feeding on C. roseus hairy roots over-expressingthe alpha or the alpha and beta subunits of anthranilate synthase.Biotechnol. Bioeng., 2006; 93: 534-540
Google Scholar - 79. Pistelli L., Giovannini A., Ruffoni B., Bertoli A., Pistelli L.: Hairyroot cultures for secondary metabolites production. Adv. Exp. Med.Biol., 2010; 698: 167-184
Google Scholar - 79. Pistelli L., Giovannini A., Ruffoni B., Bertoli A., Pistelli L.: Hairyroot cultures for secondary metabolites production. Adv. Exp. Med.Biol., 2010; 698: 167-184
Google Scholar - 80. Polivka J., Janku F.: Molecular targets for cancer therapy inthe PI3K/AKT/mTOR pathway. Pharmacol. Ther., 2014; 142: 164-175
Google Scholar - 80. Polivka J., Janku F.: Molecular targets for cancer therapy inthe PI3K/AKT/mTOR pathway. Pharmacol. Ther., 2014; 142: 164-175
Google Scholar - 81. Pollier J., Moses T., Goossens A.: Combinatorial biosynthesisin plants: a (p)review on its potential and future exploitation. Nat.Prod. Rep., 2011; 28: 1897-1916
Google Scholar - 81. Pollier J., Moses T., Goossens A.: Combinatorial biosynthesisin plants: a (p)review on its potential and future exploitation. Nat.Prod. Rep., 2011; 28: 1897-1916
Google Scholar - 82. Putalun W., Luealon W., De-Eknamkul W., Tanaka H., ShoyamaY.: Improvement of artemisinin production by chitosan in hairy rootcultures of Artemisia annua L. Biotechnol. Lett., 2007; 29: 1143-1146
Google Scholar - 82. Putalun W., Luealon W., De-Eknamkul W., Tanaka H., ShoyamaY.: Improvement of artemisinin production by chitosan in hairy rootcultures of Artemisia annua L. Biotechnol. Lett., 2007; 29: 1143-1146
Google Scholar - 83. Rahman L.U., Kitamura Y., Yamaguchi J., Mukai M., Akiyama K.,Yamamoto H., Muranaka T., Ikenaga T.: Exogenous plant H6H but notbacterial HCHL gene is expressed in Duboisia leichhardtii hairy rootsand affects tropane alkaloid production. Enzyme Microb. Technol.,2006; 39: 1183-1189
Google Scholar - 83. Rahman L.U., Kitamura Y., Yamaguchi J., Mukai M., Akiyama K.,Yamamoto H., Muranaka T., Ikenaga T.: Exogenous plant H6H but notbacterial HCHL gene is expressed in Duboisia leichhardtii hairy rootsand affects tropane alkaloid production. Enzyme Microb. Technol.,2006; 39: 1183-1189
Google Scholar - 84. Rahnama H., Hasanloo T., Shams M.R., Sepehrifar R.: Silymarinproduction in hairy root culture of Silybum marianum (L.) Gaertn.Iranian J. Biotechnol., 2008; 6: 113-118
Google Scholar - 84. Rahnama H., Hasanloo T., Shams M.R., Sepehrifar R.: Silymarinproduction in hairy root culture of Silybum marianum (L.) Gaertn.Iranian J. Biotechnol., 2008; 6: 113-118
Google Scholar - 85. Ramawat K.G., Mérillon J.M.: Natural Products: Phytochemistry,Botany and Metabolism of Alkaloids, Phenolics and Terpenes. Springer,Berlin Heidelberg 2013
Google Scholar - 85. Ramawat K.G., Mérillon J.M.: Natural Products: Phytochemistry,Botany and Metabolism of Alkaloids, Phenolics and Terpenes. Springer,Berlin Heidelberg 2013
Google Scholar - 86. Ren S., Zhang H., Mu Y., Sun M., Liu P.: Pharmacological effectsof astragaloside IV: a literature review. J. Tradit. Chin. Med., 2013;33: 413-416
Google Scholar - 86. Ren S., Zhang H., Mu Y., Sun M., Liu P.: Pharmacological effectsof astragaloside IV: a literature review. J. Tradit. Chin. Med., 2013;33: 413-416
Google Scholar - 87. Renner U.D., Oertel R., Kirch W.: Pharmacokinetics and pharmacodynamicsin clinical use of scopolamine. Ther. Drug Monit.,2005; 27: 655-665
Google Scholar - 87. Renner U.D., Oertel R., Kirch W.: Pharmacokinetics and pharmacodynamicsin clinical use of scopolamine. Ther. Drug Monit.,2005; 27: 655-665
Google Scholar - 88. Rhee H.S., Cho H.W., Son S.Y., Yoon S.Y., Park J.M.: Enhancedaccumulation of decursin and decursinol angelate in root culturesand intact roots of Angelica gigas Nakai following elicitation. PlantCell Tiss. Organ Cult., 2010; 101: 295-302
Google Scholar - 88. Rhee H.S., Cho H.W., Son S.Y., Yoon S.Y., Park J.M.: Enhancedaccumulation of decursin and decursinol angelate in root culturesand intact roots of Angelica gigas Nakai following elicitation. PlantCell Tiss. Organ Cult., 2010; 101: 295-302
Google Scholar - 89. Rudrappa T., Neelwarne B., Kumar V., Lakshmanan V.S., AswathanarayanaR.G.: Peroxidase production from hairy root cultures ofred beet (Beta vulgaris). Elect. J. Biotechnol., 2005; 8: 185-197
Google Scholar - 89. Rudrappa T., Neelwarne B., Kumar V., Lakshmanan V.S., AswathanarayanaR.G.: Peroxidase production from hairy root cultures ofred beet (Beta vulgaris). Elect. J. Biotechnol., 2005; 8: 185-197
Google Scholar - 90. Runguphan W., Qu X., O’Connor S.E.: Integrating carbon–halogenbond formation into medicinal plant metabolism. Nature,2010; 468: 461-464
Google Scholar - 90. Runguphan W., Qu X., O’Connor S.E.: Integrating carbon–halogenbond formation into medicinal plant metabolism. Nature,2010; 468: 461-464
Google Scholar - 91. Ruslan K., Selfitri A.D., Bulan S.A., Rukayadi Y., Elfahmi: Effect ofAgrobacterium rhizogenes and elicitation on the asiaticoside productionin cell cultures of Centella asiatica. Pharmacogn. Mag., 2012; 8: 111-115
Google Scholar - 91. Ruslan K., Selfitri A.D., Bulan S.A., Rukayadi Y., Elfahmi: Effect ofAgrobacterium rhizogenes and elicitation on the asiaticoside productionin cell cultures of Centella asiatica. Pharmacogn. Mag., 2012; 8: 111-115
Google Scholar - 92. Saller R., Brignoli R., Melzer J., Meier R.: An updated systematicreview with meta-analysis for the clinical evidence of silymarin.Forsch. Komplementärmed., 2008; 15: 9-20
Google Scholar - 92. Saller R., Brignoli R., Melzer J., Meier R.: An updated systematicreview with meta-analysis for the clinical evidence of silymarin.Forsch. Komplementärmed., 2008; 15: 9-20
Google Scholar - 93. Sevón N., Oksman-Caldentey K.M.: Agrobacterium rhizogenes-mediatedtransformation: root cultures as a source of alkaloids. PlantaMed., 2002; 68: 859-868
Google Scholar - 93. Sevón N., Oksman-Caldentey K.M.: Agrobacterium rhizogenes-mediatedtransformation: root cultures as a source of alkaloids. PlantaMed., 2002; 68: 859-868
Google Scholar - 94. Sharafi A., Sohi H.H., Mousavi A., Azadi P., Dehsara B., KhalifaniB.H.: Enhanced morphinan alkaloid production in hairy rootcultures of Papaver bracteatum by over-expression of salutaridinol7-o-acetyltransferase gene via Agrobacterium rhizogenes mediatedtransformation. World J. Microbiol. Biotechnol., 2013; 29: 2125-2131
Google Scholar - 94. Sharafi A., Sohi H.H., Mousavi A., Azadi P., Dehsara B., KhalifaniB.H.: Enhanced morphinan alkaloid production in hairy rootcultures of Papaver bracteatum by over-expression of salutaridinol7-o-acetyltransferase gene via Agrobacterium rhizogenes mediatedtransformation. World J. Microbiol. Biotechnol., 2013; 29: 2125-2131
Google Scholar - 95. Sharma S., Ali A., Ali J., Sahni J.K., Baboota S.: Rutin: therapeuticpotential and recent advances in drug delivery. Expert Opin. Investig.Drugs, 2013; 22: 1063-1079
Google Scholar - 95. Sharma S., Ali A., Ali J., Sahni J.K., Baboota S.: Rutin: therapeuticpotential and recent advances in drug delivery. Expert Opin. Investig.Drugs, 2013; 22: 1063-1079
Google Scholar - 96. Sharma V., Agrawal R.C.: Glycyrrhiza glabra – a plant for the future.Mintage J. Pharmaceutic. Med. Sci., 2013; 2: 15-20
Google Scholar - 96. Sharma V., Agrawal R.C.: Glycyrrhiza glabra – a plant for the future.Mintage J. Pharmaceutic. Med. Sci., 2013; 2: 15-20
Google Scholar - 97. Sharmila R., Subburathinam K.M.: Effect of signal compoundson andrographolide in the hairy root cultures of Andrographis paniculata.IJPSR, 2013, 4: 1773-1776
Google Scholar - 97. Sharmila R., Subburathinam K.M.: Effect of signal compoundson andrographolide in the hairy root cultures of Andrographis paniculata.IJPSR, 2013, 4: 1773-1776
Google Scholar - 98. Shi M., Kwok K.W., Wu J.Y.: Enhancement of tanshinone productionin Salvia miltiorrhiza Bunge (red or Chinese sage) hairy-rootculture by hyperosmotic stress and yeast elicitor. Biotechnol. Appl.Biochem., 2007; 46: 191-196
Google Scholar - 98. Shi M., Kwok K.W., Wu J.Y.: Enhancement of tanshinone productionin Salvia miltiorrhiza Bunge (red or Chinese sage) hairy-rootculture by hyperosmotic stress and yeast elicitor. Biotechnol. Appl.Biochem., 2007; 46: 191-196
Google Scholar - 99. Shilpa K., Varun K., Lakshmi B.S.: An alternate method of naturaldrug production: eliciting secondary metabolite production usingplant cell culture. J. Plant Sci., 2010; 5: 222-247
Google Scholar - 99. Shilpa K., Varun K., Lakshmi B.S.: An alternate method of naturaldrug production: eliciting secondary metabolite production usingplant cell culture. J. Plant Sci., 2010; 5: 222-247
Google Scholar - 100. Siddiqi M.H., Siddiqi M.Z., Ahn S., Kang S., Kim Y.J., SathishkumarN., Yang D.U., Yang D.C.: Ginseng saponins and the treatment ofosteoporosis: mini literature review. J. Ginseng Res., 2013; 37: 261-268
Google Scholar - 100. Siddiqi M.H., Siddiqi M.Z., Ahn S., Kang S., Kim Y.J., SathishkumarN., Yang D.U., Yang D.C.: Ginseng saponins and the treatment ofosteoporosis: mini literature review. J. Ginseng Res., 2013; 37: 261-268
Google Scholar - 101. Sivanandhan G., Rajesh M., Arun M., Jeyaraj M., Dev G.K., ManickavasagamM., Selvaraj N., Ganapathi A.: Optimization of carbonsource for hairy root growth and withaferin A and withanone productionin Withania somnifera. Nat. Prod. Commun., 2012; 7: 1271-1272
Google Scholar - 101. Sivanandhan G., Rajesh M., Arun M., Jeyaraj M., Dev G.K., ManickavasagamM., Selvaraj N., Ganapathi A.: Optimization of carbonsource for hairy root growth and withaferin A and withanone productionin Withania somnifera. Nat. Prod. Commun., 2012; 7: 1271-1272
Google Scholar - 102. Skarjinskaia M., Karl J., Araujo A., Ruby K., Rabindran S., StreatfieldS.J., Yusibov V.: Production of recombinant proteins in clonalroot cultures using episomal expression vectors. Biotechnol. Bioeng.,2008; 100: 814-819
Google Scholar - 102. Skarjinskaia M., Karl J., Araujo A., Ruby K., Rabindran S., StreatfieldS.J., Yusibov V.: Production of recombinant proteins in clonalroot cultures using episomal expression vectors. Biotechnol. Bioeng.,2008; 100: 814-819
Google Scholar - 103. Smolarczyk R., Cichoń T., Matuszczak S., Mitrus I., Lesiak M.,Kobusińska M., Kamysz W., Jarosz M., Sieroń A., Szala S.: The role ofglycyrrhizin, an inhibitor of HMGB1 protein, in anticancer therapy.Arch. Immunol. Ther. Exp., 2012; 60: 391-399
Google Scholar - 103. Smolarczyk R., Cichoń T., Matuszczak S., Mitrus I., Lesiak M.,Kobusińska M., Kamysz W., Jarosz M., Sieroń A., Szala S.: The role ofglycyrrhizin, an inhibitor of HMGB1 protein, in anticancer therapy.Arch. Immunol. Ther. Exp., 2012; 60: 391-399
Google Scholar - 104. Srivastava S., Srivastava A.K.: Hairy root culture for mass-productionof high-value secondary metabolites. Crit. Rev. Biotechnol.,2007; 27: 29-43
Google Scholar - 104. Srivastava S., Srivastava A.K.: Hairy root culture for mass-productionof high-value secondary metabolites. Crit. Rev. Biotechnol.,2007; 27: 29-43
Google Scholar - 105. Staniszewska I., Krolicka A., Malinski E., Lojkowska E., SzafranekJ.: Elicitation of secondary metabolites in in vitro cultures of Ammimajus L. Enzyme Microb. Technol. 2003; 33: 565-568
Google Scholar - 105. Staniszewska I., Krolicka A., Malinski E., Lojkowska E., SzafranekJ.: Elicitation of secondary metabolites in in vitro cultures of Ammimajus L. Enzyme Microb. Technol. 2003; 33: 565-568
Google Scholar - 106. Syklowska-Baranek K., Pietrosiuk A., Kokoszka A., FurmanowaM.: Enhancement of taxane production in hairy root culture of Taxusx media var. Hicksii. J. Plant Physiol., 2009; 166: 1950-1954
Google Scholar - 106. Syklowska-Baranek K., Pietrosiuk A., Kokoszka A., FurmanowaM.: Enhancement of taxane production in hairy root culture of Taxusx media var. Hicksii. J. Plant Physiol., 2009; 166: 1950-1954
Google Scholar - 107. Tabata H.: Production of paclitaxel and the related taxanesby cell suspension cultures of Taxus Species. Curr. Drug Targets,2006; 7: 453-461
Google Scholar - 107. Tabata H.: Production of paclitaxel and the related taxanesby cell suspension cultures of Taxus Species. Curr. Drug Targets,2006; 7: 453-461
Google Scholar - 108. Talano M.A., Oller A.L., González P.S., Agostini E.: Hairy roots,their multiple applications and recent patents. Recent Pat. Biotechnol.,2012; 6: 115-133
Google Scholar - 108. Talano M.A., Oller A.L., González P.S., Agostini E.: Hairy roots,their multiple applications and recent patents. Recent Pat. Biotechnol.,2012; 6: 115-133
Google Scholar - 109. Tang K.X., Liu D.H., Wang Y.L., Cui L.J., Ren W.W., Sun X.F.: Overexpressionof transcriptional factor ORCA3 increases the accumulationof catharanthine and vindoline in Catharanthus roseus hairyroots. Russ. J. Plant Physiol., 2011; 58: 415-422
Google Scholar - 109. Tang K.X., Liu D.H., Wang Y.L., Cui L.J., Ren W.W., Sun X.F.: Overexpressionof transcriptional factor ORCA3 increases the accumulationof catharanthine and vindoline in Catharanthus roseus hairyroots. Russ. J. Plant Physiol., 2011; 58: 415-422
Google Scholar - 110. Taylor L.P., Grotewold E.: Flavonoids as developmental regulators.Curr. Opin. Plant Biol., 2005; 8: 317-323
Google Scholar - 110. Taylor L.P., Grotewold E.: Flavonoids as developmental regulators.Curr. Opin. Plant Biol., 2005; 8: 317-323
Google Scholar - 111. Vázquez-Flota F., Hernández-Domínguez E., de Lourdes Miranda-HamM., Monforte-González M.: A differential response to chemicalelicitors in Catharanthus roseus in vitro cultures. Biotechnol.Lett., 2009; 31: 591-595
Google Scholar - 111. Vázquez-Flota F., Hernández-Domínguez E., de Lourdes Miranda-HamM., Monforte-González M.: A differential response to chemicalelicitors in Catharanthus roseus in vitro cultures. Biotechnol.Lett., 2009; 31: 591-595
Google Scholar - 112. Veerasham C.: Medicinal Plant Biotechnology. CBS Pub., 2004;New Delhi, 377-419
Google Scholar - 112. Veerasham C.: Medicinal Plant Biotechnology. CBS Pub., 2004;New Delhi, 377-419
Google Scholar - 113. Verma P.C., Singh D., Rahman L., Gupta M.M., Banerjee S.: Invitro studies in Plumbago zeylanica: rapid micropropagation and establishmentof higher plumbagin yeilding hairy root cultures. PlantPhysiol. J., 2002; 159: 547-552
Google Scholar - 113. Verma P.C., Singh D., Rahman L., Gupta M.M., Banerjee S.: Invitro studies in Plumbago zeylanica: rapid micropropagation and establishmentof higher plumbagin yeilding hairy root cultures. PlantPhysiol. J., 2002; 159: 547-552
Google Scholar - 114. Wang C.T., Liu H., Gao X.S., Zhang H.X.: Overexpression of G10Hand ORCA3 in the hairy roots of Catharanthus roseus improves catharanthineproduction. Plant Cell Rep., 2010; 29: 887-894
Google Scholar - 114. Wang C.T., Liu H., Gao X.S., Zhang H.X.: Overexpression of G10Hand ORCA3 in the hairy roots of Catharanthus roseus improves catharanthineproduction. Plant Cell Rep., 2010; 29: 887-894
Google Scholar - 115. Wasilewska A., Królicka A.: Otrzymywanie i charakterystykakultur korzeni włośnikowatych. Biotechnologia, 2005; 4: 173-188
Google Scholar - 115. Wasilewska A., Królicka A.: Otrzymywanie i charakterystykakultur korzeni włośnikowatych. Biotechnologia, 2005; 4: 173-188
Google Scholar - 116. Wongwicha W., Tanaka H., Shoyama Y., Putalun W.: Methyljasmonate elicitation enhances glycyrrhizin production in Glycyrrhizainflata hairy roots cultures. Z. Naturforsch. C, 2011; 66: 423-428
Google Scholar - 116. Wongwicha W., Tanaka H., Shoyama Y., Putalun W.: Methyljasmonate elicitation enhances glycyrrhizin production in Glycyrrhizainflata hairy roots cultures. Z. Naturforsch. C, 2011; 66: 423-428
Google Scholar - 117. Xu H., Kim Y.K., Suh S.Y., Udin M.R., Lee S.Y., Park S.U.: Deoursinproduction from hariy root culture of Angelica gigas. J. Korea Soc.Appl. Biol. Chem., 2008; 51: 349-351
Google Scholar - 117. Xu H., Kim Y.K., Suh S.Y., Udin M.R., Lee S.Y., Park S.U.: Deoursinproduction from hariy root culture of Angelica gigas. J. Korea Soc.Appl. Biol. Chem., 2008; 51: 349-351
Google Scholar - 118. Yang C., Chen M., Zeng L., ZhangL., Liu X., Lan X, Tang K., LiaoZ.: Improvement of tropane alkaloids production in hairy root culturesof Atropa belladonna by overexpressing pmt and h6h genes. PlantOmics J., 2011; 4: 29-33
Google Scholar - 118. Yang C., Chen M., Zeng L., ZhangL., Liu X., Lan X, Tang K., LiaoZ.: Improvement of tropane alkaloids production in hairy root culturesof Atropa belladonna by overexpressing pmt and h6h genes. PlantOmics J., 2011; 4: 29-33
Google Scholar - 119. Yim D., Singh R.P., Agarwal C., Lee S., Chi H., Agarwal R.: A novelanticancer agent, decursin, induces G1 arrest and apoptosis inhuman prostate carcinoma cells. Cancer Res., 2005; 65: 1035-1044
Google Scholar - 119. Yim D., Singh R.P., Agarwal C., Lee S., Chi H., Agarwal R.: A novelanticancer agent, decursin, induces G1 arrest and apoptosis inhuman prostate carcinoma cells. Cancer Res., 2005; 65: 1035-1044
Google Scholar - 120. Zang Y.X., Kim D.H., Park B.S., Hong, S.B.: Metabolic engineeringof indole glucosinolates in Chinese cabbage hairy roots expressingArabidopsis CYP79B2, CYP79B3, and CYP83B1. Biotechnol. BioprocessEng., 2009; 14: 467-473
Google Scholar - 120. Zang Y.X., Kim D.H., Park B.S., Hong, S.B.: Metabolic engineeringof indole glucosinolates in Chinese cabbage hairy roots expressingArabidopsis CYP79B2, CYP79B3, and CYP83B1. Biotechnol. BioprocessEng., 2009; 14: 467-473
Google Scholar - 121. Zhai D.D., Supaibulwatana K., Zhong J.J.: Inhibition of tumorcell proliferation and induction of apoptosis in human lung carcinoma95-D cells by a new sesquiterpene from hairy root cultures ofArtemisia annua. Phytomedicine, 2010; 17: 856-861
Google Scholar - 121. Zhai D.D., Supaibulwatana K., Zhong J.J.: Inhibition of tumorcell proliferation and induction of apoptosis in human lung carcinoma95-D cells by a new sesquiterpene from hairy root cultures ofArtemisia annua. Phytomedicine, 2010; 17: 856-861
Google Scholar - 122. Zhang H.C., Liu J.M., Lu H.Y., Gao S.L.: Enhanced flavonoidproduction in hairy root cultures of Glycyrrhiza uralensis Fisch bycombining the over-expression of chalcone isomerase gene with theelicitation treatment. Plant Cell Rep., 2009; 28: 1205-1213
Google Scholar - 122. Zhang H.C., Liu J.M., Lu H.Y., Gao S.L.: Enhanced flavonoidproduction in hairy root cultures of Glycyrrhiza uralensis Fisch bycombining the over-expression of chalcone isomerase gene with theelicitation treatment. Plant Cell Rep., 2009; 28: 1205-1213
Google Scholar - 123. Zhang L., Ding R., Chai Y., Bonfill M., Moyano E., Oksman-CaldenteyK.M., Xu T., Pi Y., Wang Z., Zhang H., Kai G., Liao Z., Sun X., TangK.: Engineering tropane biosynthetic pathway in Hyoscyamus nigerhairy root cultures. Proc. Natl. Acad. Sci. USA, 2004; 101: 6786-6791
Google Scholar - 123. Zhang L., Ding R., Chai Y., Bonfill M., Moyano E., Oksman-CaldenteyK.M., Xu T., Pi Y., Wang Z., Zhang H., Kai G., Liao Z., Sun X., TangK.: Engineering tropane biosynthetic pathway in Hyoscyamus nigerhairy root cultures. Proc. Natl. Acad. Sci. USA, 2004; 101: 6786-6791
Google Scholar - 124. Zhang L., Kai G.Y., Lu B.B., Zhang H.M., Tang K.X., Jiang J.H.,Chen W.S.: Metabolic engineering of tropane alkaloid biosynthesisin plants. J. Integr. Plant Biol., 2005; 47: 136-143
Google Scholar - 124. Zhang L., Kai G.Y., Lu B.B., Zhang H.M., Tang K.X., Jiang J.H.,Chen W.S.: Metabolic engineering of tropane alkaloid biosynthesisin plants. J. Integr. Plant Biol., 2005; 47: 136-143
Google Scholar - 125. Zhou M., Zhang C., Wu Y., Tang Y.: Metabolic engineering ofgossypol in cotton. Appl. Microbiol. Biotechnol., 2013; 97: 6159-6165
Google Scholar - 125. Zhou M., Zhang C., Wu Y., Tang Y.: Metabolic engineering ofgossypol in cotton. Appl. Microbiol. Biotechnol., 2013; 97: 6159-6165
Google Scholar - 126. Zhou M.L., Zhu X.M., Shao J.R., Tang Y.X., Wu Y.M.: Productionand metabolic engineering of bioactive substances in planthairy root culture. Appl. Microbiol. Biotechnol., 2011; 90: 1229-1239
Google Scholar - 126. Zhou M.L., Zhu X.M., Shao J.R., Tang Y.X., Wu Y.M.: Productionand metabolic engineering of bioactive substances in planthairy root culture. Appl. Microbiol. Biotechnol., 2011; 90: 1229-1239
Google Scholar