The effect of natural whey proteins on mechanisms of blood pressure regulation
Halina Car 1 , Tomasz Koprowicz 2 , Anna Tokajuk 1 , Adrian Tokajuk 1Abstract
Whey is a rich natural source of peptides and amino acids. It has been reported in numerous studies that biological active peptides isolated from cow’s milk whey may affect blood pressure regulation. Studies on animals and humans have shown that α-lactalbumin and β-lactoglobulin obtained from enzymatically hydrolysed whey inhibit angiotensin converting enzyme (ACE), while lactorphins lower blood pressure by normalizing endothelial function or by opioid receptors dependent mechanism. Whey proteins or their bioactive fragments decrease total cholesterol, LDL fraction and triglycerides, thus reducing the risk factors of cardiovascular diseases.The aim of this review is to discuss the effects of whey proteins on the mechanisms of blood pressure regulation.
References
- 1. Abubakar A., Saito T., Kitazawa H., Kawai Y., Itoh T.: Structuralanalysis of new antihypertensive peptides derived from cheese wheyprotein by proteinase K digestion. J. Dairy Sci., 1998; 81: 3131-3138
Google Scholar - 2. Ballard K.D., Bruno R.S., Seip R.L., Quann E.E., Volk B.M., FreidenreichD.J., Kawiecki D.M., Kupchak B.R., Chung M.Y., Kraemer W.J.,Volek J.S.: Acute ingestion of a novel whey–derived peptide improvesvascular endothelial responses in healthy individuals: a randomized,placebo controlled trial. Nutr. J., 2009; 8: 34
Google Scholar - 3. Chiba H., Yoshikawa M.: Bioactive peptides derived from foodproteins. Kagaku to Seibutsu, 1991; 29: 454-458
Google Scholar - 4. Faggiotto A., Paoletti R.: State-of-the-Art lecture. Statins andblockers of the renin-angiotensin system: vascular protection beyondtheir primary mode of action. Hypertension, 1999; 34: 987-996
Google Scholar - 5. Ferreira I.M., Pinho O., Mota M.V., Tavares P., Pereira A., GoncalvesM.P., Torres D., Rocha C., Teixeira J.A.: Preparation of ingredients containing an ACE-inhibitory peptide by tryptic hydrolysis of wheyprotein concentrates. Int. Dairy J., 2007; 17: 481-487
Google Scholar - 6. FitzGerald R.J., Murray B.A., Walsh D.J.: Hypotensive peptidesfrom milk proteins. J. Nutr., 2004; 134: 980S-988S
Google Scholar - 7. Fluegel S.M., Shultz T.D., Powers J.R., Clark S., Barbosa-Leiker C.,Wright B.R., Freson T.S., Fluegel H.A., Minch J.D., Schwarzkopf L.K,Miller A.J., Di Filippo M.M.: Whey beverages decrease blood pressurein prehypertensive and hypertensive young men and women. Int.Dairy J., 2010; 20: 753-760
Google Scholar - 8. Lee Y.M., Skurk T., Hennig M., Hauner H.: Effect of a milk drinksupplemented with whey peptides on blood pressure in patientswith mild hypertension. Eur. J. Nutr., 2007; 46: 21-27
Google Scholar - 9. Lv G.S., Huo G.C., Fu X.Y.: Expression of milk–derived antihypertensivepeptide in Escherichia coli. J. Dairy Sci., 2003; 86: 1927-1931
Google Scholar - 10. Madureira A.R., Pereira C.I., Gomes A.M., Pintado M.E., MalcataF.X.: Bovine whey proteins – overview on their main biological properties.Food Res. Intern., 2007; 40: 1197-1211
Google Scholar - 11. Madureira A.R., Tavares T., Gomes A.M., Pintado M.E., MalcataF.X.: Invited review: physiological properties of bioactive peptidesobtained from whey proteins. J. Dairy Sci., 2010; 93: 437-455
Google Scholar - 12. Maes W., Van Camp J., Vermeirssen V., Hemeryck M., KetelslegersJ.M., Schrezenmeir J., Van Oostveldt P., Huyghebaert A.: Influence ofthe lactokinin Ala-Leu-Pro-Met-His-Ile-Arg (ALPMHIR) on the releaseof endothelin-1 by endothelial cells. Regul. Pept., 2004; 118: 105-109
Google Scholar - 13. Mortensen L.S., Hartvigsen M.L., Brader L.J., Astrup A., SchrezenmeirJ., Holst J.J., Thomsen C., Hermansen K.: Differential effects ofprotein quality on postprandial lipemia in response to a fat-richmeal in type 2 diabetes: comparison of whey, casein, gluten and codprotein. Am. J. Clin. Nutr., 2009; 90: 41–48
Google Scholar - 14. Mullally M.M., Meisel H., Fitzgerald R.J.: Angiotensin-I-convertingenzyme inhibitory activities of gastric and pancreatic proteinasedigests of whey proteins. Int. Dairy J., 1997; 7: 299-303
Google Scholar - 15. Mullally M.M., Meisel H., FitzGerald R.J.: Identification of a novelangiotensin-I-converting enzyme inhibitory peptide correspondingto a tryptic fragment of bovine β-lactoglobulin. FEBS Lett., 1997;402: 99-101
Google Scholar - 16. Mullally M.M., Meisel H., FitzGerald R.J.: Synthetic peptides correspondingto alpha-lactalbumin and beta-lactoglobulin sequenceswith angiotensin I-converting enzyme inhibitory activity. Biol.Chem., 1996; 377: 259-260
Google Scholar - 17. Murakami M., Tonouchi H., Takahashi R., Kitazawa H., Kawai Y.,Negishi H., Saito T.: Structural analysis of a new anti–hypertensivepeptide (β-lactosin B) isolated from a commercial whey product. J.Dairy Sci., 2004; 87: 1967-1974
Google Scholar - 18. Nagaoka S., Kanamaru Y., Kuzuya Y., Kojima T., Kuwata T.: Comparativestudies on the serum cholesterol lowering action of wheyprotein and soy protein in rats. Biosci. Biotechnol. Biochem., 1992;56: 1484-1485
Google Scholar - 19. Nurminen M.L., Sipola M., Kaarto H., Pihlanto-Leppala A.,Piilola K., Korpela R., Tossavainen O., Korhonen H., Vapaatalo H.:α-lactorphin lowers blood pressure measured by radiotelemetryin normotensive and spontaneously hypertensive rats. Life Sci.,2000; 66: 1535-1543
Google Scholar - 20. Ohlsson L., Hertervig E., Jönsson B.A., Duan R.D., Nyberg L.,Svernlov R., Nilsson A.: Sphingolipids in human ileostomy contentafter meals containing milk sphingomyelin. Am. J. Clin. Nutr., 2010;91: 672-678
Google Scholar - 21. Pal S., Ellis V.: The chronic effects of whey proteins on bloodpressure, vascular function, and inflammatory markers in overweightindividuals. Obesity, 2010; 18: 1354-1359
Google Scholar - 22. Pal S., Ellis V., Dhaliwal S.: Effects of whey protein isolate onbody composition, lipids, insulin and glucose in overweight andobese individuals. Br. J. Nutr., 2010; 104: 716-723
Google Scholar - 23. Pal S., Ellis V., Ho S.: Acute effects of whey protein isolate oncardiovascular risk factors in overweight, post-menopausal women.Atherosclerosis, 2010; 212: 339-344
Google Scholar - 24. Pihlanto-Leppälä A., Koskinen K., Piilola T., Tupasela T., KorhonenH.: Angiotensin I-converting enzyme inhibitory properties ofwhey protein digests: concentration and characterization of activepeptides. J. Dairy. Res., 2000; 67: 53-64
Google Scholar - 25. Pihlanto-Leppälä A., Rokka T., Korhonen H.: Angiotensin I convertingenzyme inhibitory peptides derived from bovine milk proteins.Intern. Dairy J., 1998; 8: 325-331
Google Scholar - 26. Pins J.J., Keenan J.M.: Effects of whey peptides on cardiovasculardisease risk factors. J. Clin. Hypertens., 2006; 8: 775-782
Google Scholar - 27. Pins J.J., Keenan J.M.: The antihypertensive effects of a hydrolysedwhey protein isolate supplement (BioZate®1). Cardiovasc.Drugs Ther., 2002; 16: 68
Google Scholar - 28. Rombaut R., Camp J., Dewettinck K.: Analysis of phosphor – andsphingolipids in dairy products by a new HPLC method. J. Dairy Sci.,2005; 88: 482-488
Google Scholar - 29. Seppo L., Jauhiainen T., Poussa T., Korpela R.: A fermented milkhigh in bioactive peptides has a blood pressure-lowering effect inhypertensive subjects. Am. J. Clin. Nutr., 2003; 77: 326-330
Google Scholar - 30. Sipola M., Finckenberg P., Vapaatalo H., Pihlanto-Leppala A., KorhonenH., Korpela R., Nurminen M.L.: α-lactorphin and β-lactorphinimprove arterial function in spontaneously hypertensive rats. LifeSci., 2002; 71: 1245-1253
Google Scholar - 31. Tavares T., Contreras M.M., Amorim M., Pintado M., Recio I.,Malcata F.X.: Novel whey-derived peptides with inhibitory effectagainst angiotensin–converting enzyme: in vitro effect and stabilityto gastrointestinal enzymes. Peptides, 2011; 32: 1013-1019
Google Scholar - 32. Vermeirssen V., Deplancke B., Tappenden K.A., Van Camp J.,Gaskins H.R., Verstraete W.: Intestinal transport of the lactokininAla-Leu-Pro-Met-His-Ile-Arg through a Caco-2 Bbe monolayer. J.Pept. Sci., 2002; 8: 95-100
Google Scholar - 33. Yamauchi R., Usui H., Junden J., Takenaka Y., Tani F., YoshikawaM.: Characterization of β-lactotensin, a bioactive peptide derivedfrom bovine β-lactoglobulin, as a neurotensin agonist. Biosci. Biotechnol.Biochem., 2003; 67: 940-943
Google Scholar - 34. Zhang X., Beynen A.: Lowering effect of dietary milk-whey proteinv. casein on plasma and liver cholesterol concentrations in rats.Br. J. Nutr., 1993; 70: 139-146
Google Scholar - 35. Zimecki M., Artym J.: Właściwości terapeutyczne białek i peptydówz siary i mleka. Postępy Hig. Med. Dośw., 2005; 59: 309-323
Google Scholar